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Analysis of Fetal and Placental Development in Rats after Administration of Hydroalcoholic Extract from the Root of Petiveria alliacea L. (Phytolaccaceae) International Journal of Morphology
Maia,Carina Scanoni; Teixeira,Valéria Wanderley; Teixeira,Álvaro Aguiar Coelho; Pontes Filho,Nicodemos Teles.
We investigated the effect of hydroalcoholic extract the Petiveria alliacea root on fetal and placental development female rats pregnancy. Thirty female rats were mated and divided in groups: I - rats treated with placebo and sacrificed on the 7th day; II - rats treated with hydroalcoholic extract and sacrificed on the 7th day; III - rats treated with placebo and sacrificed on the 14th day; IV - rats treated with hydroalcoholic extract and sacrificed on the 14th day; V - rats treated with placebo for analysis of the offspring; VI - rats treated with hydroalcoholic extract for analysis of the offspring. The extract was administered by gavage in the dosage of 18mg/kg body wt., in 5th day of pregnancy. The implantation sites and placenta were fixed in Bouin...
Tipo: Journal article Palavras-chave: Petiveria alliacea; Uterus; Placenta; Hydroalcoholic extract.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100023
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Bovine embryo induces an anti-inflammatory response in uterine epithelial cells and immune cells in vitro: Possible involvement of interferon tau as an intermediator OAK
Talukder, Anup K.; Yousef, Mohamed S.; Rashid, Mohammad B.; Awai, Kensuke; Acosta, Tomas J.; Shimizu, Takashi; Okuda, Kiyoshi; Shimada, Masayuki; Imakawa, Kazuhiko; Miyamoto, Akio.
Recent observations suggest that the bovine uterus starts to react to the early embryo immediately after its arrival from the oviduct. The present study aimed to investigate the effect of the early developing embryo on the immune-related gene profile in bovine uterine epithelial cells (BUECs) in vitro, and to further examine the impact of conditioned media (CM), either from embryo-BUEC co-culture or embryo culture alone, on gene expression in peripheral blood mononuclear cells (PBMCs). First, BUECs were co-cultured with morulae (n = 10) for D5-D9 (D0 = IVF), and gene expression in BUECs was analyzed. Subsequently, PBMCs were cultured in CM from embryo-BUEC co-culture or D5-D9 embryo culture, and gene expression was evaluated. In BUECs, the embryo induced...
Palavras-chave: Bovine; Embryo; Immunity; Interferon tau (IFNT); Uterus.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4532
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Ca2+ entry, efflux and release in smooth muscle Biol. Res.
MATTHEW,A; SHMYGOL,A; WRAY,SUSAN.
Control of smooth muscle is vital for health. The major route to contraction is a rise in intracellular [Ca2+], determined by the entry and efflux of Ca2+ and release and re-uptake into the sarcoplasmic reticulum (SR). We review these processes in myometrium, to better understand excitation-contraction coupling and develop strategies for preventing problematic labours. The main mechanism of elevating [Ca2+] is voltage-gated L-type channels, due to pacemaker activity, which can be modulated by agonists. The rise of [Ca2+] produces Ca-calmodulin and activates MLCK. This phosphorylates myosin and force results. Without Ca2+ entry uterine contraction fails. The Na/Ca exchanger (NCX) and plasma membrane Ca-ATPase (PMCA) remove Ca2+, with contributions of 30%...
Tipo: Journal article Palavras-chave: Uterus; SR; Signalling; Ca-ATPase.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400017
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CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice BJMBR
Araújo,J.M.D.; Silva,L.A.S.; Felix,F.B.; Camargo,E.A.; Grespan,R..
Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 receptor plays an important role in eosinophil migration to the mouse uterus was confirmed, because we observed reduction in eosinophil peroxidase activity in these antagonist-treated uteruses. The antagonist also influenced uterine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Estradiol; Chemokine receptor; Immunology; Menopause; Uterus.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100603
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Differences in progesterone concentrations and mRNA expressions of progesterone receptors in bovine endometrial tissue between the uterine horns ipsilateral and contralateral to the corpus luteum OAK
TAKAHASHI, Hiroto; HANEDA, Shingo; KAYANO, Mitsunori; MATSUI, Motozumi; 羽田, 真悟; 茅野, 光範; 松井, 基純.
Because the establishment of pregnancy begins at the uterine horn ipsilateral to the corpus luteum (ipsi-horn) in cattle, levels of progesterone (P4) and receptor expression in the endometrial tissue, which regulate the intrauterine environment for embryo development, may differ between the ipsi-horn and the uterine horn contralateral to corpus luteum (contra-horn). The aim of the present study was to determine the endometrial tissue P4 concentrations and nuclear progesterone receptor (PGR), progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 mRNA expressions in the cranial and middle parts of the uterine horns during the luteal phase. The results showed higher endometrial tissue P4 concentrations in the cranial part of the ipsi-horn than in...
Palavras-chave: Bovine; Progesterone; Receptor; Uterus.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4364
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Diffuse heterotopic hair in a canine uterus: case report Arq. Bras. Med. Vet. Zootec.
Malm,C.; Serakides,R.; Silva,J.F.; Nascimento,E.F.; Boeloni,J.N.; Viana,F.A.B.; Souza,E.M.; Ocarino,N.M..
The present report describes the clinical and pathological findings related to diffuse heterotopic hairs associated with pseudo-placentational endometrial hyperplasia in a canine uterus. Macroscopically, the uterus was intensely enlarged and thicker, and the endometrial surface was irregular and covered with black hairs similar to the hairs of the bitch. Histologically, heterotopic hairs associated with pseudo-placentational endometrial hyperplasia were observed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Hair; Heterotopia; Uterus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500003
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Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus Anais da ABC (AABC)
GOSSLER,VANESSA S.A.; SANTOS,FRANCISLAINE A.G.; AZEVEDO,ALINE R. DE; GONÇALVES,PRISCILA C.; RIGOLO,HUGO A.; TREVISAN,CLEONICE; MASSENO,ANA PAULA B.; CARDOSO,ANA PAULA M.M.; PAPA,PAULA C.; CASTILHO,CALIÊ; GIOMETTI,INES C..
ABSTRACT This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p<0.05). The correlation between endometrial thickness and uterine cell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AgNOR; Dogs; Hematoxylin-eosin; Uterus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401719
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Experimental use of fibrin glue derived from snake venom in non-pregnant canine uterus J. Venom. Anim. Toxins incl. Trop. Dis.
Moraes,J.R.E.; Correia,P.H.A.; Camplesi,A.C.; Moraes,F.R..
This study evaluated the use of fibrin glue derived from snake venom in the healing process after canine hysterorrhaphy. Three groups of four animals were submitted to uterine hysterotomy followed by wound closure. In Group 1, double-layer suture was used, the first with Schimieden pattern, the second with Cushing pattern; in Group 2, only fibrin glue; and in Group 3, the same as for Group 1 but with fibrin glue as suture reinforcement. Results indicated that fibrin glue produced less inflammation in the exudative phase, and exacerbated deposition of connective tissue and angiogenesis in the proliferative and maturation phases of the healing process, favoring its evolution.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Healing; Fibrin glue; Uterus; Surgical adhesives; Wound closure.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200003
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Expression of mdr isoforms in mice during estrous cycle and under hormone stimulation Genet. Mol. Biol.
Schiengold,Marion; Schwantes,Lavínia; Ribeiro,Maria F.; Lothhammer,Nívia; Gonzalez,Tatiana P.; Chies,Jose Artur Bogo; Nardi,Nance B..
The multidrug resistance (MDR) phenotype is associated with the expression of P-glycoprotein (Pgp), coded by the multigenic mdr family. Mice present the isoforms mdr1 and mdr3, which are responsible for multidrug resistance, and mdr2, that is involved in the transport of phospholipids. mdr1 expression has more recently been associated also with the secretion of steroid hormones. This work presents an RT-PCR analysis of the expression of mdr isoforms, in several organs of mice during different phases of the estrous cycle. Additionally, females were ovariectomized, submitted to different hormone treatments, and their uterus was analyzed for the expression of mdr isoforms. The results show that in the adrenal gland and ovaries mdr1 is the main isoform during...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adrenal gland; Multidrug resistance; Ovaries; Steroids; Uterus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400029
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Expression of Uterine Sensitization-Associated Gene-1 (USAG-1) in the Mouse Uterus During the Peri-Implantation Period OAK
MAEDA, Kanami; LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志.
Rat uterine sensitization-associated gene-1 (USAG-1) mRNA is expressed in the uterus during the peri-implantation period, and its mRNA expression in uterine epithelial cells is highest on day 5 of pregnancy. On the other hand, since changes in USAG-1 mRNA expression in the mouse uterus are not seen during the estrous cycle, USAG-1 expression might be specifically regulated by embryonic factors rather than by the maternal environment. However, the expression pattern and function of USAG-1 in the mouse uterus have not been determined. Thus, we examined the tissue-specific USAG-1 mRNA expression in the uteri of ICR mice during peri-implantation using real-time quantitative PCR. Uterine tissues, such as the myometrium, luminal epithelium, and stroma, were...
Palavras-chave: Implantation; Mouse; MRNA; Uterine sensitization-associated gene-1 (USAG-1); Uterus.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3024
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Histological Remarks of the Uterus of Sympterygia acuta (Garman, 1877) and Sympterygia bonapartii (Müller &amp; Henle, 1841) (Chondrichthyes; Rajidae) International Journal of Morphology
Díaz-Andrade,María Constanza; Lopez-Cazorla,Andrea; Galíndez,Elena Juana.
Chondrichthyes constitute a conserve and vulnerable clade of increasing importance. However, there is scarce knowledge about the morphological aspects of the reproduction, so that only one work exists about the structure of the gravid uterus of an oviparous species. The work presented here describes the morphological, histological and histochemical remarks of the uterus of two Rajids of the Sympterygia genus along the life cycle. Both species presented paired uteri that lead independently to a common urogenital sinus. The uteri of immature females are filiform, translucent and unspecialized tubes. When sexual development starts, all the genital system displays a transitional and progressive change. In all studied females, independently of the maturing...
Tipo: Journal article Palavras-chave: Chondrichthyes; Rajidae; Uterus; Sympterygia.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300014
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Histometric Parameters of Uterus and Vagina in Breeder Female Rabbits on the Day After Weaning According to the Ordinal Number of Parturitions International Journal of Morphology
Banda-Herrera,José Alfonso; Aragón Hernández,Jesús; López Ordaz,Reyes; Hernández Espinosa,Jorge; Pérez-Martínez,Mario.
The objective of this study was to establish in New Zealand female rabbits, the effect of ordinal number of parturitions on some histological parameters on the day after weaning. Tissue fragments of uterus and vagina were obtained from females of first, second and third parturitions and were processed imbedding them in paraffin in order to do histological cuts. In eight microscope fields captured by a camera connected to an optic microscope, the lumen and glandular endometrial epithelium height, as well as the thickness of vaginal and myometrium epithelium thickness were measured. From the results that were obtained it was concluded that there are significant differences (p<0.05) in histological parameters evaluated in uterus and vagina between does of...
Tipo: Journal article Palavras-chave: Histometry; Uterus; Vagina; Weaning; Rabbit Doe.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200058
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Intra-uterine experimental infection by Ureaplasma diversum induces TNF-α mediated womb inflammation in mice Anais da ABC (AABC)
Silva,Jamile R.; Ferreira,Lício F.A.A.; Oliveira,Percíllia V.S.; Nunes,Ivanéia V.; Pereira,Ítalo S.; Timenetsky,Jorge; Marques,Lucas M.; Figueiredo,Tiana B.; Silva,Robson A.A..
Ureaplasma diversum is an opportunistic pathogen associated with uterine inflammation, impaired embryo implantation, infertility, abortions, premature birth of calves and neonatal pneumonia in cattle. It has been suggested that the intra-uterine infection by Ureaplasma diversum can cause vascular changes that hinder the success of pregnancy. Thus, the aim of this study was to evaluate the changes of intrauterine site of A/J mice in estrus or proestrus phase inoculated with Ureaplasma diversum. The infection was monitored at 24, 48 and 72 hours by the PCR methodology to detect the Ureaplasma in the inoculation site and the profile of circulating blood cells. Morphological changes, intensity of inflammation and the production of cytokines were compared. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; Uterus; Ureaplasma; Mice.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200643
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Laparoscopic-assisted treatment of pyometra associated with mammary fibroadenomatous hyperplasia in a cat Ciência Rural
Colomé,Lucas Marques; Kunert Filho,Hiran Castagnino; Feranti,João Pedro Scussel; Santos,Fabiane Reginatto dos; Sartori,Luana Walendorff; Brun,Maurício Veloso.
This paper describes a case of laparoscopic-assisted ovariohysterectomy in a female cat presenting pyometra and mammary fibroadenomatous hyperplasia. Using four portals, mesovarium were ligated by titanium ligature clips whereas the uterine vessels were occluded by video-assisted conventional ligatures. There were no postoperative complications. Video-assisted technique can be an alternative method for treatment of pyometra and cystic endometrial hyperplasia in female cats.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laparoscopy; Ovariohysterectomy; Endosurgery; Progesterone; Uterus.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000300018
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Morphological Changes in the Pregnant Uterus of the Smooth Hound Dogfish Mustelus schmitti Springer, 1939 (Gatuzo) (Condrichthyes, Triakidae): Microscopic Study and Phylogenetic Reproductive Implications International Journal of Morphology
Galíndez,E. J; Díaz Andrade,M. C; Moya,A. C; Estecondo,S.
Elasmobranchs are important, not only as an economic resource but also for their phylogenetic position. Their slow growth and long reproductive cycles, together with the high fisheries pressure exerted, attempt to the knowledge of all biological parameters of species. In this work we analyze the histology of the pregnant uterus of the gatuzo, a viviparous aplacental shark. The mucosa depicts modifications similar to those of placental species and others proper of histotrophism. Likewise, a network-like muscular layer in the mucosa was described for first time in the genus. The presence of vitelline plates in the gut of at term embryos suggests that the maternal supplies are not enough for the big fetus development. The relationships between maternal...
Tipo: Journal article Palavras-chave: Elasmobranchs; Reproduction; Uterus; Viviparity; Mustelus schmitti.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400004
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Multiple congenital defects in a newborn foal Arq. Bras. Med. Vet. Zootec.
Silva,J.F.; Serakides,R.; França,S.A.; Boeloni,J.N.; Ocarino,N.M..
A case of multiple congenital defects in a newborn foal is reported. The animal showed hypoplasia of the left pelvic limb bones, uterus unicornis, congenital diaphragmatic hernia, and unilateral renal and ureteral agenesis. This report includes the macroscopic and microscopic lesions observed in the case.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Foal; Congenital defects; Uterus; Kidney; Diaphragm.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601671
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The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats International Journal of Morphology
Yang,Zhi; Xiao,Ke; Wang,Wei; Tang,Juan; Sun,Peng-Peng; Peng,Ke-Mei; Song,Hui.
The present study was to investigate the effects of visfatin on the morphological structure and function of the rat uterus during inflammation. The expression and distribution of visfatin, morphological structure, eosinophils (EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induced rat were studied using hematoxylin-eosin staining (HE), immunohistochemical methods, western blots and enzyme-linked immunosorbent assay (ELISA). The present study showed that visfatin positive cells dispersed widely in the uterus, and strong positive staining was observed mainly in the cell cytoplasm. Compared with saline group, in visfatin group, more uterine glands were found, EOS increased, and the difference was significant (P<0.05), MPO reduced, and...
Tipo: Journal article Palavras-chave: Visfatin; LPS-induced rat; Uterus; Inflammation; Cytokine.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100031
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Uterine leiomyoma in chimpanzee (Pan troglodytes) Arq. Bras. Med. Vet. Zootec.
Silva,A.E.; Ocarino,N.M.; Cassali,G.D.; Nascimento,E.F.; Coradini,M.A.; Serakides,R..
Uma chimpanzé de 22 anos de idade foi necropsiada com histórico clínico de anorexia, vômitos freqüentes e desidratação conseqüentes à gastrite iatrogênica. Macroscopicamente, o útero apresentava-se aumentado de volume, com o lúmen totalmente ocluído por nódulos firmes, esbranquiçados e coalescentes que se estendiam para o miométrio. Histologicamente, os nódulos eram constituídos por leiomiócitos bem diferenciados dispostos em várias direções e com coloração característica pelo tricrômio de Gomori e Masson. Pela imunoistoquímica, as células neoplásicas apresentavam marcação forte e difusa de receptores para progesterona e estrógeno, assim como de actina alfa de músculo liso. Algumas células neoplásicas e o estroma apresentavam marcação para vimentina e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chimpanzee; Uterus; Leiomyoma; Immunohistochemical.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000100020
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